WebThe inulinase gene from Kluyveromyces marxianus (KmINU), with the ability of converting inulin to fructose, was introduced into S. cerevisiae D452-2. The inulinase gene was fused … WebAug 21, 2014 · The inulinase gene was fused to three different types of promoter (GPD, PGK1, truncated HXT7) and secretory signal sequence (KmINU ... The inulin fermentation performance of the nine transformants containing different promoter and signal sequence combinations for inulinase production were compared to select an optimized expression ...
An engineered cryptic Hxt11 sugar transporter facilitates glucose ...
WebNov 3, 2011 · The promoter (b) is preferably selected from HXT7, truncated HXT7, PFK1, FBA1, PGK1, ADH1 and TDH3. In a preferred embodiment, the nucleic acid expression construct with which a cell according to the invention is transformed is a nucleic acid molecule according to the invention, as defined herein and above. WebMay 2, 2024 · Therefore, we hypothesized that when the N‐terminus of Hxt2 is replaced for the corresponding region of Hxt11, stable membrane expression of Hxt2 might be obtained even at high glucose concentration when the gene is expressed under control of the constitutive truncated HXT7 promoter that was also used in previous studies (Shin et al., … pokemon brilliant diamond save file location
Optimizing promoters and secretory signal sequences for …
Webfor HXT3, HXT6, HXT7, and GAL2 exhibited hexose-inhibitable 73As(OH) 3 uptake, whereas a strain lacking all 18 hexose transport-related genes (HXT1 to HXT17 and GAL2), FPS1 and ACR3, exhibited <10% of wild type 73As(OH) 3 transport. When HXT1, HXT3, HXT4, HXT5, HXT7,orHXT9was individually expressed in that strain, hexose-inhibitable 73As(OH) 3 ... Webthemevalonatepathway(MVA),byseveral strategies including: overexpressinga truncated andthussolubleas wellasnon-regulatedversion ofHMG-CoAreductase(tHMG1)[4,5], down-regulating squalene synthase (ERG9),which leadsviaseveral stepstothemajor end pokemon brilliant diamond scyther evolution