Orbitally shaken

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August undefined, 2024

WebJun 14, 2024 · Cells were orbitally shaken at 180 rpm at 37°C with 5% CO 2 and 85% humidity in ISF-4-W incubators (Kühner, Birsfelden, Switzerland). Cells were seeded at 0.5 … WebNov 1, 2024 · Here, we set up a scalable process for AAV production, using orbitally shaken bioreactors and a fully characterized suspension-adapted cell line, HEKExpress. We conducted a proof-of-concept ...

Effect of baffle structure on flow field characteristics of orbitally ...

WebOrbitally shaken bioreactors (OSBs) utilize the same hydrodynamic principles that define the success of smaller-scale cultur … Increasing the cultivation volume from small to large … WebNov 20, 2024 · Disposable orbitally shaken bioreactors have been widely used for mammalian cell culture in suspension. Three kinds of baffle structures: vertical baffle, inclined baffle and horizontal baffle were designed in this work. The flow fields of the shaking bioreactor with different baffle structures were … fme topologybuilder

Disposable 600-mL orbitally shaken bioreactor for mammalian cell …

WebSep 1, 2014 · Study of the phase-resolved flow characteristics allowed to built a Re-Fr map, where four quadrants associated to different flow regimes are identified: in-phase toroidal vortex (low Fr and high Re), out-of-phase precessional vortex (high Fr and high Re), in-phase single vortex (low Fr and low Re), out-of-phase counter-rotating toroidal vortex … WebExhibitor. Kuhner shaker, founded in 1949 in Basel, Switzerland, is a science-first shaker manufacturer renowned worldwide for our uncompromising shakers, incubator shakers, and orbitally shaken bioreactors complying with GMP regulations. From bench top shakers to large scale industrial shaking machines, we offer machines of the highest quality. WebDec 20, 2024 · Orbitally shaken platforms can accommodate a wide range of vessel types and sizes, yielding a large degree of flexibility and independence from specific … fme to pl259

Orbitally shaken bioreactors—viscosity effects on flow …

Determination of a scale-up factor from mixing time studies in ...

WebAug 19, 2024 · The orbitally shaken bioreactors (OSRs) were shaken on a platform connected to a shaker motor. The distance between the attachment point of the platform and the center axis of the shaker motor represents half the shaking diameter (= displacement radius). The OSRs contained deionized water maintained at 37 °C. WebJul 15, 2013 · Orbitally shaken bioreactors (OSRs) support the efficient cultivation of mammalian cells in suspension at various scales of operation. Here we introduce a disposable culture vessel with a ventilated cap having a nominal volume of 600 mL (TubeSpin ® bioreactor 600) for medium-scale cell cultivation. It has a conical bottom … fme to bnc fme to ts9

"WebMar 1, 2024 · Orbitally shaken bioreactors (OSRs) is one of important bioreactors for mammalian cells cultivation in suspension, especially for the screening of valuable … " - Orbitally shaken

Orbitally shaken

WebThe Boekel Scientific Orbital and Reciprocating Shaking Water Bath is designed for a wide range of temperature-controlled shaking protocols, such as Northern and Southern … WebJul 20, 2024 · In this study, we carried out the present numerical simulation to first characterize the performance of two different shaking methodologies in an orbitally shaken bioreactor, namely, one...

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WebNov 21, 2024 · Here, we report the implementation of a scalable process for the production of AAV vectors using suspension HEKExpress cells in orbitally shaken bioreactors (OSRs) and polyethylenimine (PEI)-mediated transient transfection. WebMar 24, 2024 · We present a new theoretical model describing gravity–capillary waves in orbitally shaken cylindrical containers. Our model can account for both one-layer free …

WebDec 20, 2024 · In this section, we will present the operation of shake-tube (Fig. 1) and benchtop (Fig. 2) bioreactors in perfusion mode.In the case of the orbitally shaken tubes (OrbShake tubes), we distinguish two different experiments referred to as VCD max and VCD SS experiment. The first one evaluates the maximum achievable VCD for a given … WebDec 2, 2013 · Among disposable bioreactor systems, cylindrical orbitally shaken bioreactors show important advantages. They provide a well-defined hydrodynamic flow combined with excellent mixing and oxygen transfer for mammalian and plant cell cultivations.

WebThe shaker (25 mL–6 L) was the most widely used orbitally shaken bioreactor (OSB) type, providing a cost-effective system that is effective for a broad range of applications … WebThis study investigated the efficacy of a novel correlation of power input, energy dissipation rate and mixing time as a potential route to identify the orbitally shaken bioreactor (OSB) system. The Buckingham’s π-theorem was used to designate and transform dimensionless Newton numbers with five relevant power input variables. These variables …

WebDec 20, 2024 · An orbitally shaken bioreactor (OSB) is a culture vessel, which is placed on an orbitally shaken platform (e.g., a shaker table in an incubator shaker). The shaking diameter is different and often adjustable.

WebNov 15, 2010 · In orbitally shaken bioreactors, the container moves with the shaker, inducing liquid motion through contact with the walls. This mixing system is the same regardless of the scale of operation. Thus, similar mixing principles can be expected at different scales. This may explain why the mixing time did not increase with the scale and why the ... greensboro youth council santas workshopWebJan 15, 2014 · At laboratory scale, cells are usually grown in low shear devices, with orbitally shaken bioreactors (OSRs) being largely employed in the early stages of bioprocess … greensboro youth for christWebAn Erlenmeyer flask, also known as a conical flask (British English) or a titration flask, is a type of laboratory flask which features a flat bottom, a conical body, and a cylindrical neck. It is named after the German chemist … fme to smaWebThe orbitally shaken bioreactors (OSB) from Kuhner are ideally suited for the cultivation of mammalian and human cell cultures as well as insect and plant cells. The bioreactors are … fme trim charactersWebJan 22, 2010 · An overview of the disposable bioreactors that are currently commercially available is provided, and the domination of wave-mixed, orbitally shaken, and, in particular, stirred disposable bioreactors in animal cell-derived productions at … fme to ch239WebAug 11, 2024 · Orbitally shaken bioreactors (OSRs) support the suspension cultivation of animal cells at volumetric scales up to 200 L and are a potential alternative to stirred-tank … fme to sharepointWebThe SB10-X is an orbital shaken benchtop bioreactor system for the cultivation of human, mammalian and plant cells in a single-use bag. It has a working volume from 4 litres up to … fme turn off feature caching