Cytoplasmic asters
WebCytoplasmic asters were localized by staining with an anti-a-tubulin antibody. Enucle-ation of MII oocytes caused no significant change in the number of cytoplasmic asters. WebFeb 1, 2005 · Cytoplasmic asters did not duplicate and were clearly seen at the surface of the parthenogenetic embryo until third and fourth mitoses (Figs. 8C, D). Download : …
Cytoplasmic asters
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WebAug 15, 2003 · Tubulin staining reveals that the asters assemble spontaneously only in the anterior region of the parthenogenetic egg, whereas the remaining cytoplasm is devoid of these structures (Fig. 1F–H).We found only two exceptional eggs in which the asters form through the whole cytoplasm among all the parthenogenetic eggs scored (n = … WebThe cytoplasmic asters became undetectable within 2 h of oocyte activation, irrespective of the presence or absence of the donor chromosomes. After the standard NT protocol, a …
Webarray of MTs organizes in clear and visible compartments that we call yolk cytoplasmic layer asters (YCL asters) (Fig. 1C–G), with MTs radially orienting from each of the domain’s center. To ... WebPurification of meiotic spindles and cytoplasmic asters from mouse oocytes The unfertilized mouse oocyte is arrested at second metaphase of meiosis with microtubules existing exclusively in the meiotic spindle. Multiple inactive cytoplasmic microtubule organizing centers (MTOCs) are also present.
WebNov 11, 2011 · However, recent work has shown that they do assemble multiple acentriolar MTOCs prior to NEB. 68 On average, ∼80 MTOCs or asters form in each oocytes; these form both out in the cytoplasm and along the nuclear surface. The cytoplasmic asters were observed to migrate toward the nuclear region, and both cytoplasmic and nuclear … WebJan 1, 2012 · At the time of nuclear envelope breakdown (NEBD), the asters on the pronuclear surface massively polymerise microtubules toward the chromosomes due to Ran GTPase, whereas the cytoplasmic asters become destabilised and lose their polymerised microtubule signal, pointing toward an inactivation of their MTOC function.
WebRemarkably, the asters have been observed only in the anterior cytoplasm of the parthenogenetic eggs (Riparbelli and Callaini, 2003; Fig. 8 C and D). Aster formation …
WebNov 14, 2000 · Numerous cytoplasmic asters appeared at the cortex of both fertilized (not shown) and unfertilized eggs at the end of meiosis II (compare (i,j), which show meiosis II and the end of meiosis, respectively). (m–p) In unfertilized eggs, the asters migrated towards the female pronucleus and two associated with it (arrowheads in (o)) while the … how to stop dogs jumping fencesWebUnlike the oocytes of most other animal species, unfertilized murine oocytes contain cytoplasmic asters, which act as microtubule-organizing centers following fertilization. This study examined the role of asters during the first cell cycle of mouse nuclear transfer (NT) embryos. NT was performed by intracytoplasmic injection of cumulus cells. Cytoplasmic … how to stop dogs licking woundsWebFeb 16, 1999 · Thus, the sperm centrosome must have recruited γ-tubulin from the maternal cytoplasm and gained the ability to nucleate Mts and form asters after entry into the oocyte. Surprisingly, 20 min after fertilization, during metaphase of meiosis I, neither sperm asters nor γ-tubulin staining was found associated with sperm nuclei (Fig. 1 C ). reactive forms in angular validationWebMay 10, 2002 · The bacterium Wolbachia manipulates reproduction in millions of insects worldwide; the most common effect is cytoplasmic incompatibility (CI). We found that CI … reactive forms validation in angularWebBackground: Cytasters (cytoplasmic asters) are centriole-based nucleation centers of microtubule polymerization that are observable in large numbers in the cortical cytoplasm of the egg and zygote of bilaterian organisms. In both protostome and deuterostome taxa, cytasters have been described to develop during oogenesis from vesicles how to stop dogs from licking youWebNov 1, 2003 · So the cytoplasmic asters as well as nucleation of microtubules from the spindle poles is solely due to DOC1R depletion. Altogether, our experiments demonstrate that DOC1R regulates microtubule organization at least in metaphase II (see Discussion). The depletion of DOC1R induces severe damage to the microtubule cytoskeleton, which … how to stop dogs licking pawsWebJan 1, 2012 · The cores of these asters are composed of a few PCM components, including pericentrin and gamma-tubulin. Based on this composition and the ability of these … reactive foveolar changes