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Binary vector pcambia-1300

WebSDS for the pCambia vectors are available by request from Marker Gene Technologies, Inc. Shipping and Storage The plant expression vectors are shipped at room temperature. Store all samples of the vector at –20 C or below, once resuspended. Products are guaranteed for six months from date of shipment when stored properly. Contents WebA binary vector was invented soon after it had been elucidated that crown gall tumorigenesis was caused by genetic transformation of plant cells with a piece of DNA, T-DNA for transferred DNA,...

Agrobacterium tumefaciens- - ScienceDirect

WebSep 8, 2014 · The fragment was then ligated with a GFP-containing pCAMBIA 1300 binary vector to develop the vector named as pDsTGFP (Figure 1B). The vector has two AhdI sites at the 13 th and 1329 th position, respectively (Figure 1B). Thus, the 3′ T-overhang ends can be generated by digesting the pDsTGFP vector with the enzyme AhdI (Figure … WebpCAMBIA has wide range of vector like 1300,2300,2301. it is binary vector kanamycin based with small replicon and has plant selection … sharp book for men https://remax-regency.com

pCambia1301 Plant Expression Vector (ab275753) Abcam

http://www.biovector.net/product/1319.html WebJul 24, 2013 · A twin T-DNA system is a convenient strategy for creating selectable marker-free transgenic plants. The standard transformation plasmid, pCAMBIA 1300, was modified into a binary vector consisting of two separate T-DNAs, one of which contained the hygromycin phosphotransferase ( hpt) marker gene. WebSep 8, 2014 · In this study, a novel binary T-vector was constructed by integrating two AhdI restriction sites into the backbone vector pCAMBIA 1300. The T-vector also contains a … sharp books

Transfer DNA binary system - Wikipedia

Category:A novel binary T-vector with the GFP reporter gene for …

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Binary vector pcambia-1300

Conferring DNA virus resistance with high …

WebAug 14, 2015 · Grains of an insect resistant transgenic Bt rice (Oryza sativa L. Japonica cv Ariete), transformed by Agrobacterium tumefaciens with the binary vector pCAMBIA 1300 containing the synthetic Cry1B gene from Bacillus thuringiensis, and its corresponding wild-type (WT) were used in this study . The CRM of the GM maize MON863 9.85 % (ERM … WebJul 1, 2024 · The pCAMBIA 1300 plasmid containing a GFP reporter gene driven by the Super promoter and harboring a Hygromycin resistance gene was used for transformation of somatic embryos. ... we transfected embryogenic calli with A. tumefaciens strain GV3101 containing a pCAMBIA 1300 binary vector, ...

Binary vector pcambia-1300

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WebpCAMBIA2301 Agrobacterium binary vector for plant transformation, with kanamycin-resistance and GUS genes. Sequence Author: Cambia Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7k Plasmids: Plant Vectors More Plasmid Sets Home Plasmids Plant VectorspCAMBIA2301 Show … WebThe vector pCAMBIA- 1300: SniOLP was digested with Eco RI enzyme and made blunt ended by end filling. This blunt-ended vector was used for cloning the blunt-ended Rs-AFP2 expression cassette.

WebJun 1, 2007 · The cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants. WebJun 9, 2024 · In the first step, an EcoRI/KpnI fragment of ~ 2.4 kb was excised from the plasmid pKSMYMIV-A and cloned into the EcoRI/KpnI sites of binary vector pCAMBIA-1300 (Cambia) to produce the recombinant plasmid pCAM-MYMIV-AM.

WebpCAMBIA1302. Agrobacterium binary vector for plant transformation, with hygromycin- and kanamycin-resistance and GFP genes. Sequence Author: Cambia. Open in … WebEnter the email address you signed up with and we'll email you a reset link.

WebThe cDNA was cloned into a binary vector (pCAMBIA 1300) and transformed into indica rice var Pusa Basmati-1. Southern hybridization analysis of transgenic rice plants revealed stable integration of the Sod1 transgene in the rice genome. The mRNA transcript of Sod1 was detected by Northern hybridisation in the transgenic rice plants.

WebSep 16, 2008 · The first T-DNA of the pCAMBIA 1300 vector contained the hygromycin phosphotransferase (hpt) selectable marker gene, while the second T-DNA carried the β … sharp bookWebApr 7, 2024 · How to order pCAMBIA 1300 vector? Question. 3 answers. Apr 3, 2024; ... The steps include cloning your gene into a binary vector, transferred the vector into Agrobacterium, ... sharp book a repairWebSep 21, 2024 · A genetically engineered chimeric virus crTMV-CP-PLRV composed of the crucifer-infecting tobacco mosaic virus (crTMV) RNA and the potato leafroll virus (PLRV) coat protein (CP) was obtained by agroinfiltration of Nicotiana benthamiana with the binary vector pCambia-crTMV-CPPLRV. The significant levels of the chimeric virus enabled … sharp bookshelfWebJun 1, 2024 · The new plant RNAi binary vector, named pCAMBIA2301-GW-RNAi, has two inverted repeated Gateway cassettes driven by the cauliflower mosaic virus 35S (CaMV … sharp boob painWebDec 18, 2008 · Vector Construction A transformation vector was constructed and denoted pCHO. It was derived from the Agrobacterium tumefaciens binary vector pCAMBIA 1300 ( http://www.cambia.org.au) by replacement of the original hph cassette driven by the 35S promoter from CaMV with newly constructed cassettes. sharp bosch microwave mitbbsWebthe binary vector pCambia 1300-Rb:: uidA to replace the uidA gene and generate pCambia 1300-Rb::MCM2 fragment2. The M2-sense1 primer allowed us to add an XbaI restriction site at the 5 ′ end of the PCR product, and M2-antisense1 was designed downstream of the internal XbaI (1844 bp down-stream of the start codon) site. The … sharp boomboxes w/cdWebJun 19, 2007 · Biological Sciences Two type III effector genes of Xanthomonas oryzae pv. oryzae control the induction of the host genes OsTFIIA γ 1 and OsTFX1 during bacterial blight of rice Akiko Sugio, Bing Yang, Tong Zhu, and Frank F. White Authors Info & … sharp boomerang